目錄:愛必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs01082Recombinant Rat IFN-γ
| 供貨周期 | 一周 | 規(guī)格 | 20ug |
|---|
| 概述 | |
|---|---|
| 種屬 | Rat |
| 形態(tài) | Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and 0.05 % Tween-80. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC |
| 內(nèi)毒素水平 | Less than 0.1 EU/µg of rRtIFN-γ as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Accession # | P01581 |
| 預(yù)期分子量 | Approximay 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 97 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 25712 |
| 參考文獻(xiàn) | |
|---|---|
| 1. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.<br/>2. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.<br/>3. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, <br/>4. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55. | |
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